Why I Love Science

It's the closest thing I could find in the real world to being on Star Trek.

 

Education

University of Pittsburgh, August 2012-August 2018

Doctor of Philosophy in Molecular, Cellular, and Developmental Biology. Minor in Teaching.

 

Messiah College, August 2006-May2010 Summa Cum Laude

Bachelor of Science in Biology. Bachelor of Arts in English

Appointments (which could be things like research tech positions, postbaccs, etc):

Core Technician II, October 2011 - July 2012

Lonza Inc, Walkersville MD

 

Pilot Product Technician I, August 2010 - October 2011

MedImmune LLC, Gaithersburg MD

 

STEP Student, Summers 2007 - 2009

US Army Research Laboratory, Adelphi MD

 

 

Publications

Nadel, C.M., Mackie, T.D., and Gardner, R.G. (In revision) Osmolyte accumulation regulates the SUMOylation and inclusion dynamics of the Cyc8-Tup1 transcriptional corepressor. PLoS Genetics

 

Hager, N.A., Krasowski, C.J., Mackie T.D., Kolb A.K., Needham P., Augustine, A.A., Dempsey A., Szent-Gyorgyi C., Bruchez M., Bain D., Kwiatkowski, A.V., O’Donnell, A.F., and Brodsky, J.L. (2018) Select α-arrestins control the cell surface abundance of the mammalian Kir2.1 protein in a yeast model.  Journal of Biological Chemistry, 293(28) 11006-11021.

 

Mackie, T.D. and Brodsky, J.L. (2018) Investigating Potassium Channels in Budding Yeast: A Genetic Sandbox. Genetics, 209(3) 637-650.

 

Mackie, T.D., Kim B.Y., O’Donnell, A.F., Subramanya, A.R., Bain, D., Welling, P.A., and Brodsky, J.L. (2018) The endosomal trafficking factors CORVET and ESCRT negatively regulate plasma membrane residence of the Renal Outer Medullary Potassium channel (ROMK).  Journal of Biological Chemistry, 293(9) 3201-3217.

 

O’Donnell, B.M., Mackie, T.D., Subramanya, A.R., and Brodsky, J.L. (2017) Endoplasmic Reticulum Associated Degradation of the Renal Outer Medullary Potassium Channel, ROMK, Underlies Type II Bartter Syndrome.  Journal of Biological Chemistry, 292(31), 12813-12827.

 

O’Donnell, B.M., Mackie, T.D., and Brodsky, J.L. (2017) Linking channelopathies with endoplasmic reticulum associated degradation. Channels (Austin).  Jul 19:1-3.

 

Guerriero, C.J., Reutter, K.R., Augustine, A.A., Preston, G.M., Weiberth, K.F., Mackie, T.D., Cleveland-Rubeor, H.C., Bethel, N.P, Callenberg, K.M., Nakatsukasa, K., Grabe, M, and Brodsky, J.L. (2017) Transmembrane helix hydrophobicity is an energetic barrier during the retrotranslocation of integral membrane ERAD substrates. Molecular Biology of the Cell. 28(15), 2076-2090.

 

Finch, A.S., Mackie, T.D., Sund, C.J. and Sumner, J.J. (2011).  Metabolite analysis of Clostridium acetobutylicum: Fermentation in a microbial fuel cell. Bioresource Technology, 102(1), 312-315.

 

 

Research Interest

 

 

Honors and awards

Andrew Mellon Predoctoral Fellowship, September 2017-April 2018

Margaret C. Oweida Predoctoral Fellowship, April 2017

APS Meritorious Research Award, April 2017

Departmental Honors, Messiah College Department of English, May 2010

First Place, US Army Research Laboratory Student Research Symposium, August 2009

 

Selected publications